ACKGROUND: Mefentrifluconazole and triticonazole are the triazole fungicide. The maximum residue levels for agricultural product will be set up. Therefore, development of the official analytical method for determination of mefentrifluconazole and triticonazole residues from agricultural crops was necessary due to safety management, and then a simultaneous analytical method was developed for the determination of mefentrifluconazole and triticonazole (triazole fungicide) in agricultural crops .
METHODS AND RESULTS: Samples were extracted using acetonitrile and purified using dispersive solid phase extraction, and then detected with liquid chromatograph-tandem mass spectrometry (LC-MS/MS). Matrix-matched calibration curves (0.0025-0.25 μg/mL) were linear into a sample extract with r2>0.99. For validation, the recovery test was carried out at three fortification levels (LOQ, 10 LOQ and 50 LOQ) from agricultural samples. The results for mefentrifluconazole and triticonazole were ranged between 92.3 to 115.3% and 91.4 to 108.5%, and RSD (relative standard deviation) values were also below than 6.0%. Furthermore, inter-laboratory was conducted to validate the method.
CONCLUSION: All values were corresponded with the criteria ranges requested in both the CODEX (CAC/GL 40-1993, 2003) and MFDS guidelines(2016). Therefore, the proposed method can be used as an official analytical method for determination of mefentrifluconazole and triticonazole (triazole fungicide) in the Republic of Korea.