BACKGROUND: G protein coupled receptors (GPCRs) are widely distributed in various organisms, from bacteria to mammalian. Insect GPCRs shown as in vertebrate GPCRs are membrane receptors that coordinates or involve in various physiological process such as learning/memory, development, locomotion, circadiac rhythm, and reproduction, etc. This study aimed to identify GPCRs expressed in fat body and examined the expression pattern of GPCRs in different temperature conditions.
METHODS AND RESULTS: To identify GPCRs genes in different temperature conditions, total RNA of fat body in Plutella xyostella larva were extracted and the transcriptomes have been analyzed via next generation sequencing method. From the fat body transcriptomes, GPCR Family A, B, and F were identified such as opsin, gonadotropin-releasing hormone receptor, neuropeptide F receptor, muthuselah (Mth), diuretic hormone receptor, and frizzled etc. Expressions of GPCRs in the fat body at high or low temperature such as serotonin receptor, rhodopsin sexpeptide receptor, Mth-like 3 were lower than those of the control (25℃) whereas expressions of C-C chemokine receptor, frizzled-4 and stan were higher.The phylogenetic analyses were performed to investigat their relationship among identified GPCRs.
CONCLUSION: Transcriptome of fat body can provide understanding the pools of GPCRs. Identifications of fat body GPCRs can provide new targets for the control of insect pests.